Welcome to the Advanced Cell Engineering and 3D Models Core at Baylor College of Medicine.
Continuing the tradition of innovative collaboration with Baylor College of Medicine, the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation has generously donated $2.26 million to enable the establishment of the Cell-Based Assay Screening Service (C-BASS) core facility, housed in the Verna and Marrs McLean Department of Biochemistry and Molecular Biology. By providing access to professional expertise and a variety of cutting-edge technologies and robotics platforms, the C-BASS core facility seeks to facilitate the advancement of our understanding of complex biological questions using well-established model systems, and to foster collaborations amongst BCM researchers.
The three major platforms are RNAi-mediated functional genomics, BiFC-based protein-protein interaction studies, and CRISPR/Cas9-mediated genome editing.
The C-BASS core houses several genomic libraries, including human and mouse shRNA and cDNA library collections. shRNA/cDNA clones are provided individually, in arrayed format, or as pools. The automated instruments in the core facilitate the use of these reagents in screens and in studies of specific pathways or individual genes.
Services and Resources:
Read core guidelines and use the online forms on the Request Services tab to get started. (One-time registration is required to set up your account.) Please send all inquiries to cbasscore@bcm.edu |
Hugo Villanueva, Ph.D.
Email: hugov@bcm.edu
Tel: (713) 798-0118
Jun Xu, Ph.D.
Email: junx@bcm.edu
Tel: (713) 798-8987
Fax: (713) 796-9438
Location |
Hours |
Advanced Cell Engineering and 3D Models Core Room T143, One Baylor Plaza, Baylor College of Medicine, Houston, TX 77030 Phone: 713-798-8987 Fax: 713-796-9438 email: ATC-ACE3MCore@bcm.edu |
9 a.m. - 5 p.m., Monday to Friday
|
Visit our BCM core website for additional information.
Scientific publications containing data obtained using the services offered by the core should include the following statement in the acknowledgment section: This project was supported by the Advanced Cell Engineering and 3D Models Core at BCM.
Name | Role | Phone | Location | |
---|---|---|---|---|
Hugo Villanueva |
Director
|
713-798-0118
|
hugov@bcm.edu
|
Room T143
|
Jun Xu |
Director
|
713-798-8987
|
junx@bcm.edu
|
Room T143
|
Internal BCM iLab Support |
Internal BCM iLab Support
|
ilabs-support@bcm.edu
|
Price Schedule |
► CRISPR Cloning Services (4) | |||
Name | Description | Price | |
---|---|---|---|
CRISPR/Cas9 vectors |
|
Inquire | |
CRISPR gRNA Vector Construction (sequence-verified) |
|
Inquire | |
Cas9/gRNA Cleavage Verification Service - T7 endonuclease assay (per guide) |
We will test the efficiency of cleavage in 293T or relevant cells. Service includes transfection, PCR, and analysis. |
Inquire | |
Complete Vector Cloning Package (sequence and cleavage verified vectors) |
We will design and construct 3-4 guide RNA vectors for your gene of interest, and test their cleavage efficiency in 293T cells. |
Inquire | |
► shRNA / cDNA Library Collections (7) | |||
Name | Description | Price | |
Individual shRNA Clones - glycerol stock (per clone; 96-well plate format) | Inquire | ||
Individual cDNA and GIPZ shRNA Clones - glycerol stock (per clone; in tubes) |
Clones are distributed as individual glycerol stocks in tubes. If you order >30 clones, we highly recommend arraying the clones in 96-well plates, which is also cheaper per clone. Please see custom library for pricing. |
Inquire | |
GIPZ Non-Silencing Control - glycerol stock (per clone) | Inquire | ||
GIPZ shRNA Positive Controls - GADPH and EGS (glycerol stock)(per clone) | Inquire | ||
Empty pGIPZ Vector - glycerol stock (per clone) | Inquire | ||
Custom shRNA/cDNA Libraries in 96-well plates (glycerol stock, per clone) |
The core assembles in 96-well plates shRNA/cDNA clones based on users' requests |
Inquire | |
Pre-assembled GIPZ shRNA GO Libraries in 96-well format (glycerol stock)(per clone) |
Kinome and Phosphatome sub-libraries |
Inquire | |
► Lentiviral Stocks (3) | |||
Name | Description | Price | |
Lentivirus and retrovirus packaging (DNA prepared by core) (8mls) |
|
Inquire | |
Lentivirus and retrovirus packaging (DNA provided by user) (8mls) |
|
Inquire | |
Lentivirus and retrovirus packaging in 96-well format (per plate) |
Viruses will be packaged in 96-well plates (2 plates for each set) ~100ul/well virus, unfiltered, untitered |
Inquire | |
► Other (2) | |||
Name | Description | Price | |
Stable cell line generation (including virus packaging) |
We will package viruses and transduce your cell line of choice to generate stable cell lines
|
Inquire | |
Electroporation (w/o optimization) | Inquire |