About

Mouse Metabolism Core (MERGED)

Our iLab page moved but not the physical location of the core.  It is now Mouse Metabolism & Phenotyping core. Please use the URL below for iLab request. 

https://bcm.corefacilities.org/sc/2937/mouse-metabolism-and-phenotyping-core/?tab=services

 

 

Please bring your mice to the satellite facility located on the 6th floor of ABBR Building, Room 632 for metabolism work.

 

 

 

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Overview

The Mouse Metabolism Core facility under the Diabetes Research Center is one out of 11 NIH-funded core facilities throughout the United States that provides research support to the investigators. This core serves new and established investigators working in the field of diabetes and metabolic diseases. The core also offers extensive support to other investigators wishing to address diabetes-related hypotheses in terms of metabolic disorders and diabetes utilizing mouse models.

Services

Cellular Oxygen Consumption and Glycolysis Monitoring Using Extracellular Flux Analyzer (XF24) From Seahorse Bio. Measure mitochondrial respiration in intact skeletal muscle fibers, fatty acid oxidation in C2C12 myocytes, glycolysis and oxidative metabolism in cancer cells, understand the relationship between bioenergetic rates, ATP turnover and more.

Comprehensive Laboratory Animal Monitoring System (CLAMS). Simultaneous monitoring of food intake, energy expenditure and real-time body temperature (surgery required for temp.) by measuring VO2, VCO2, RQ (respiratory quotient) and heat production.

Euglycemic Hyperinsulinemic Clamp. Hepatic glucose production, glucose disposal rate, glucose infusion rate, glycogen synthesis and tissue specific glucose uptake using conscious mouse.

Other services include:

  • Long term infusion through jugular vein in non-sedated mouse for metabolic studies
  • MRI - body composition analysis of conscious mice
  • Mouse activity monitoring - total activity including horizontal activity, distance travel, rest time by beam block technique in home cage
  • GTT - intra peritoneal glucose tolerance test and oral GTT
  • ITT - insulin tolerance test
  • In vivo lipolysis
  • Lipid profile measurement - VLDL, HDL, LDL
  • Routine blood parameter check - includes glucose, insulin, cholesterol, leptin, adiponectin, TG, free fatty acid, glycerol
  • Freshly isolated primary hepatocytes
  • Freshly isolated islets
  • Lipid extraction from various tissue
  • TLC run for lipid assay

The core also provides help in data analysis and scientific explanation with proper discussion related to the metabolic changes.

Leadership

Director
Dr. Pradip Saha
Phone: (713) 798-3704
Email: mmc-derc@bcm.edu

 

Technician
Luge Li
Phone: (713) 798-6686
Email: mmc-derc@bcm.edu

Location and hours of operation

The core is located on the 6th floor of the Margaret Alkek Building for Biomedical Research, Room R630.

Hours of operation are 9:30 a.m. to 5 p.m.

Links

Contacts

Name Role Phone Email Location
Pradip Saha
Director
 
713-798-3704
 
psaha@bcm.edu
 
R-630
 
Internal BCM iLab Support
Internal BCM iLab Support
 

 
ilabs-support@bcm.edu
 

 

Services


Search available services: View: by category alphabetically
► Glucose Metabolism (5)
► Body Composition (2)
► Metabolic Monitoring (6)
► Extracellular Flux Analyzer (XF-24) from Seahorse Bio. (1)
► Hyperinsulinemic Clamp (7)
► Lipid (6)
► Diabetes & Lipid Panel (10)
► Basic Biochemistry / Kidney / Electrolytes Panel (8)
► Plasma Liver Panel (6)
► Lipid Extraction (1)
► TLC for Lipid (1)
► Primary Cell Culture (3)
► Tail Vein Injection (1)
► Long term jugular infusion (1)
► Miscellaneous (1)