Neurovisualization Core

Overview of Services

Neuropathology Core:

The Neuropathology Core provides the capability to perform structural studies of the brain. It provides users anatomical expertise in preparing and analyzing developing and adult brains for deviations from typical structure in species ranging from rodents through to primates, including human.

• Tissue processing, embedding and sectioning
• Routine Histology (H&E, Nissl, Fast Blue and other histological stain)
• Immunostaining (IHC, IF)
• Golgi-Cox staining
• X-gal staining

Microscopy Core:

The Microscopy Core is currently equipped with confocal and two-photon microscopes. The role of the core is to provide imaging capabilities for investigators to conduct experiments in both infixed tissue and anaesthetized live animals.
Our instruments could be used for following functions, such as, Optical sectioning of fluorescent tissue using apotome, confocal or two-photon imaging. Super-resolution optical sectioning of fluorescent tissue using STED3X Technique. Time-lapse imaging and /or electrical recording of living tissue or cell culture. Tile scanning and stitching of composite images. 3D reconstruction of biological tissue/cells.In vivo time-lapse imaging of live animals.FRAP, FRET, Bleaching.Stereology using Stereo Investigator system. Matlab, Imaris and Neurolucida data analysis.

Instruments

1. Zeiss 880 Airyscan confocal microscope system
2. Zeiss 710 confocal microscope system
3. Leica TCS SP8 confocal microscope system
4. Leica TCS SP8X confocal microscope system
5. Leica TCS SP8X STED3x super-resolution microscope system
6. Nikon Ti2E Spinning Disc confocal microscope
7. Zeiss 7MP two-photon system
8. Zeiss fluorescence Apotome microscope with Stereo Investigator system for stereology
9. three computer workstations installed with imaris and /or Neurolucida software

Please make acknowledgement in your publication as follwoing.

“The project was supported in part by Baylor College of Medicine IDDRC Grant Number P50HD103555 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development for use of the Microscopy Core facilities”

Leadership

Neuropathology Core:

Roy Sillitoe, Ph.D. | Director
(832) 824-8913
sillitoe@bcm.edu

Microscopy Core: 

Dinghui Yu, Ph.D. | Director
(832) 824-8790
dyu@bcm.edu

Location and hours of operation

Links and Resources

1. Neuropathology Core TCH site: https://nri.texaschildrens.org/core-facilities/neuropathology-core
2. Neuropathology Core BCM site: https://www.bcm.edu/research/centers/intellectual-developmental-disabilities/cores/neuropathy
3. Microscopy Core: Procedure for required laser test
4. Microscopy Core TCH site:https://nri.texaschildrens.org/core-facilities/microscopy-core
5. Microscopy Core IDDRC site: https://www.bcm.edu/research/centers/intellectual-developmental-disabilities/cores/microscopy-core

Contacts